Western blot was performed using RIG-I Polyclonal Antibody (Product # PA5-79148) and a 120 kDa band corresponding to RIG-I was observed across cell lines and tissue extracts tested except Mouse Heart which is reported to be low. The expression of protein was also increased upon Lipopolysaccharide treatment. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with LPS (1 ug for 18 Hours) (Lane 2), Raw 264.7 (Lane 3), tissue extracts of Mouse Heart (Lane 4), Mouse Spleen (Lane 5) and Mouse Lung (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).